Effect of GM plants on mutualistic soil fungal communities assessed by DNA- and RNA- based pyrosequencing and molecular fingerprinting
نویسندگان
چکیده
Cultivation of Genetically Modified (GM) plants has increased significantly over the last decades. GM traits may negatively affect beneficial soil fungi, such as Arbuscular Mycorrhizal (AM) fungi, potentially leading to alterations in soil functioning. Here, we test two Bt-Maize varieties for their effects on soil AM fungal communities, targeting both DNA and RNA as an inclusive way of detecting community trends, by 1) 454 pyrosequencing using general fungal rRNA gene-directed primers and 2) and AM fungi-specific molecular T-RFLP profiling, in a pot experiment. Potential GM-induced effects were then compared to the normal natural variation of AM fungal communities in the field. AM fungi were found to be abundant in soil, accounting for 30% and 8% of total recovered DNA-and RNA-derived fungal sequences, respectively, after 104 days of plant growth implying that AM fungi are an abundant and active functional group in soil. Pyrosequencing and T-RFLP generally agreed with respect to the main trends observed, and RNA-and DNA-based sequence analyses yielded most of the same AM fungal lineages. No significant differences were detected between AM fungal communities associated with GM versus non-GM plants. Our approach of comparison against " natural " field communities confirmed that GM-related variation was lower than seasonal and between-field variation.
منابع مشابه
Tracking Fungal Community Responses to Maize Plants by DNA- and RNA-Based Pyrosequencing
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